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Characterization and stability of extracellular alkaline proteases from halophilic and alkaliphilic bacteria isolated from saline habitat of coastal Gujarat, India BJM
Dodia,Mital S.; Joshi,Rupal H.; Patel,Rajesh K.; Singh,Satya P..
The present study deals with the isolation and characterization of the moderately halophilic-alkaliphilic bacteria from a saline habitat in western India. Eight different bacterial strains were isolated using enrichment techniques at 20% (w/v) NaCl and pH 10. The isolates exhibited diversity towards gram's reaction, colony and cell morphology. They were able to grow and produce alkaline protease over a broad range of NaCl, 5-20% (w/v) and pH, 8-10. None of the isolates could grow at pH 7, and one could not grow even at pH 8. Crude and partially purified proteases from strain S5 were subjected to characterization with reference to pH, salt stability and protein folding. Optimum protease activity and stability was recorded at 10% salt and pH 9-9.5....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Halophiles; Alkaliphiles; Alkaline protease; Protease stability; Protein folding; Extremophiles.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300015
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Extremely thermophilic microorganisms and their polymer-hidrolytic enzymes Rev. Microbiol.
Andrade,Carolina M.M.C.; Pereira Jr.,Nei; Antranikian,Garo.
Thermophilic and hyperthermophilic microorganisms are found as normal inhabitants of continental and submarine volcanic areas, geothermally heated sea-sediments and hydrothermal vents and thus are considered extremophiles. Several present or potential applications of extremophilic enzymes are reviewed, especially polymer-hydrolysing enzymes, such as amylolytic and hemicellulolytic enzymes. The purpose of this review is to present the range of morphological and metabolic features among those microorganisms growing from 70oC to 100°C and to indicate potential opportunities for useful applications derived from these features.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Archaea; Extremophiles; Amylases; Xylanases; Pullulanases; Thermostability.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400001
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Microbial Diversity and Biosignatures: An Icy Moons Perspective ArchiMer
Jebbar, Mohamed; Hickman-lewis, Keyron; Cavalazzi, Barbara; Taubner, Ruth-sophie; Rittmann, Simon K.-m. R.; Antunes, Andre.
The icy moons of the outer Solar System harbor potentially habitable environments for life, however, compared to the terrestrial biosphere, these environments are characterized by extremes in temperature, pressure, pH, and other physico-chemical conditions. Therefore, the search for life on these icy worlds is anchored on the study of terrestrial extreme environments (termed “analogue sites”), which harbor microorganisms at the frontiers of polyextremophily. These so-called extremophiles have been found in areas previously considered sterile: hot springs, hydrothermal vents, acidic or alkaline lakes, hypersaline environments, deep sea sediments, glaciers, and arid areas, amongst others. Such model systems and communities in extreme terrestrial environments...
Tipo: Text Palavras-chave: Extremophiles; Prokaryotes; Metabolism; Diversity; Adaptation; Space explorat.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00606/71771/70275.pdf
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Proteobacteria, extremophiles and unassigned species dominate in a tape-like showerhead biofilm BJM
Charnock,Colin; Nordlie,Anne-Lise.
Abstract The development of showerhead biofilms exposes the user to repeated contact with potentially pathogenic microbes, yet we know relatively little about the content of these aggregates. The aim of the present study was to examine the microbial content of tape-like films found protruding from a domestic showerhead. Culturing showed that the films were dominated by aerobic α- and β-proteobacteria. Three isolates made up almost the entire plate count. These were a Brevundimonas species, a metalophilic Cupriavidus species and a thermophile, Geobacillus species. Furthermore, it was shown that the Cupriavidus isolate alone had a high capacity for biofilm formation and thus might be the initiator of biofilm production. A clone library revealed the same...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Showerhead biofilm; Extremophiles; Unassigned sequences; Biofilm-formation potential.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200345
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Thermosipho spp. Immune System Differences Affect Variation in Genome Size and Geographical Distributions ArchiMer
Haverkamp, Thomas H. A.; Geslin, Claire; Lossouarn, Julien; Podosokorskaya, Olga A.; Kublanov, Ilya; Nesbo, Camilla L..
Thermosipho species inhabit thermal environments such as marine hydrothermal vents, petroleum reservoirs, and terrestrial hot springs. A 16S rRNA phylogeny of available Thermosipho spp. sequences suggested habitat specialists adapted to living in hydrothermal vents only, and habitat generalists inhabiting oil reservoirs, hydrothermal vents, and hotsprings. Comparative genomics of 15 Thermosipho genomes separated them into three distinct species with different habitat distributions: The widely distributed T. africanus and the more specialized, T. melanesiensis and T. affectus. Moreover, the species can be differentiated on the basis of genome size (GS), genome content, and immune system composition. For instance, the T. africanus genomes are largest and...
Tipo: Text Palavras-chave: Extremophiles; Hydrothermal vents; Mobile DNA; Speciation; Thermotogae; Vitamine B-12.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00478/58934/61503.pdf
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Worms’ Antimicrobial Peptides ArchiMer
Bruno, Renato; Maresca, Marc; Canaan, Stéphane; Cavalier, Jean-françois; Mabrouk, Kamel; Boidin-wichlacz, Céline; Olleik, Hamza; Zeppilli, Daniela; Brodin, Priscille; Massol, François; Jollivet, Didier; Jung, Sasha; Tasiemski, Aurélie.
Antimicrobial peptides (AMPs) are natural antibiotics produced by all living organisms. In metazoans, they act as host defense factors by eliminating microbial pathogens. But they also help to select the colonizing bacterial symbionts while coping with specific environmental challenges. Although many AMPs share common structural characteristics, for example having an overall size between 10–100 amino acids, a net positive charge, a γ-core motif, or a high content of cysteines, they greatly differ in coding sequences as a consequence of multiple parallel evolution in the face of pathogens. The majority of AMPs is specific of certain taxa or even typifying species. This is especially the case of annelids (ringed worms). Even in regions with extreme...
Tipo: Text Palavras-chave: Antibiotics; Annelids; Nematodes; AMP; Extremophiles.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00511/62307/66556.pdf
Registros recuperados: 6
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